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Welcome to DUVASU, Mathura

   

Pharmacology & Toxicology

Department
of Veterinary Pharmacology & Toxicology

Department
of Materia Medica earlier, later Pharmacology and now Pharmacology and
Toxicology was established as an independent Department in 1949. It was
the first department in the country to start postgraduate degree programme
(MVSc) in 1962 and later PhD in 1970.

Since
inception, department has been pioneer in undertaking research on
indigenous plants and more than ninety plants have been screened for
different pharmacological activities and toxicity potential.
Some of these plants have been included in pharmaceutical formulations by
certain companies. Andranchne cordifloia (Bhatoola) leaves, Acacia
leucophloea (Raunja) tender leaves and pods and Naruydia medagascariensis
leaves have been reported for the first time to contain quite high levels
of hydrocyanic acid and responsible for toxicity in sheep and goats.
Treatment of poisoning with Paspalum scorbiculatum (Kodo) was also
suggested for the first time by this Department.

Studies
on characterization of autonomic and autacoids receptors in goat uterus,
ureter and oviduct and buffaloes uterus, ureter and oesophagus have been
conducted. Tocolytic efficacy of selective b2 adrenoceptor agonists,
calcium channel blockers and non-specific smooth muscle relaxants in
buffaloes was evaluated and reported to possess promising therapeutic
potential for clinical use in veterinary medicine. Recently, using
pharmacological and molecular biology tools, different types of potassium
channels have been characterized in buffalo uterus, involvement of
different isoforms of sodium pump in regulating sheep coronary and
pulmonary arteries tone and its modulation by fatty acids has been
elucidated. Further research work on functional and molecular regulation
of vascular potassium channels in sepsis-induced oxidative stress in rats
and modulation of myometrial spontaneity by sodium pump and endogenous
nitric oxide in buffaloes is in progress.

Disposition
kinetic data on ofloxacin has been generated in sheep, goats, cow and
buffalo calves and neonatal cow calves to ensure its rational and safe
use. For complete treatment of nematodiasis in goats, suitable dose
requirement of fenbendazole has been suggested. Flumethrine pour-on (1%
w/v) was found to be very effective against Damalinia caprae infestation
in goats and the single application was observed to be effective for 42
days as it prevented the re-infestation during this period. Recently,
Department also reported a very rapid and simple fluorimetric assay method
for estimation of ciprofloxacin in plasma. Buffalo perimetrial uterine
strips has been found to be very sensitive to histamine and can be used
for bioassay of histamine. Department has also published a book of
“Veterinary Toxicology”.

The
department is having well equipped laboratories to undertake research on
neuro and autonomic pharmacology, pharmacokinetics, medicinal plants and
environmental pollutants. Some of the major equipments which are available
in the laboratories are data acquisition system based physiograph, Millar
pressure transducer, spectrofluorometer, spectrophotometer,
plethysmometer, hot-cold plate, analgesiometer, aggressometer, pole
climbing apparatus, rota-rod, soxhlet apparatus, rotary vacuum evaporator,
BOD incubator, biosafety cabinet etc. Further, CIL with sophisticated
equipments like HPLC, Atomic absorption spectrophotometer and several
others has further improved the scope for hands on training students to
postgraduate students.

Department
has an excellent organizational capacity. Recent successful organization
of VIII ISVPT conference in November 2008 is testimony to the capability
and strength of Department; and in coming six months, Department will be
organizing two training programmes of six days duration each for imparting
hands on training to postgraduate students and young faculty members from
other Departments of the country.

Coming soon ........

Research

RESEARCH/INFRASTRUCTURE  DEVELOPMENT PROJECTS

a.      
ICAR
Niche
Area of Excellence PROGRAMME (2011-12)         
             
:          
Rs. 467.00 lacs

b.     
Rashtriya
Krishi
Vikas Yojana Project (2010-11)                        
              
 
:          
Rs. 125.00 lacs

c.      
ICAR
Outreach
Programme on Ethnoveterinary Medicine            
(2009-10):
         
Rs. 80.00 lacs

 

INTERNATIONAL RECOGNITION OF RESEARCH WORK AND
CITATIONS IN BOOKS

 a.      
Several
research papers published in International journals of repute have
citations in
different books of Pharmacology, Toxicology and Medicine published by
IOWA
State University Press, IOWA, Butterworth Heinemann, Boston, Book Power
formerly ELST with Saunders.

 

b.     
Research
paper on “Cellular coupling of
potassium channels with beta2
adrenoceptors in mediating myometrial relaxation in buffaloes (Bubalus
bubalis)”
authored by Soumen Choudhury et
al.,
 published
in Journal of Veterinary Pharmacology and Therapeutics. [2010,
33(1):22-7]
cited in

a.      
Membrane
Glycoproteins: Advances in Research
and Application” (2011)
published by Scholarly Editions,
Atalnta,
Georgia.

b.     
Issues in
Veterinary Practices and
Specialties” ( 2011)
published by Scholarly Editions, Editor:
Q Ashton Acton, (ISBN:
978-1-464-96586-9)

c.      
Research
paper on “
Eicosapentaenoic acid-induced
endothelium-dependent
and –independent relaxation of sheep pulmonary artery” authored by
Thakur uttam
Singh et al., published in European Journal of Pharmacology(Cardiovascular
Pharmacology),
636:108-113 cited in

a.      
Nitrogen Oxides: Advances in Research and
Application (2011) Q. Ashton Acton (Ed). Scholarly Editions, Atalnta,
Georgia.

b.     
Bioactive Food as Dietary Interventions for
Cardiovascular Disease (2012). Ronald Ross Watson, Victor R. Preedy
(Ed). Academic
Press, San Diego, USA. (ISBN 978-0-12-396-485-4)

 

PATENTS
FILED BY THE
DEPARTMENT

S. No.

Application No.

Title

1

2539/DEL/2012 16/08/2012

Novel herbal oxytocic for treatment of uterine
inertia in animals

2

103/DEL/2014 15/01/2014

Novel herbal antimicrobial gel (DUVATOP-G) for
animals

3

2642/DEL/2014 
15/09/2014

Non-estrogenized and consistent rat uterus model
for pharmacodynamic studies on myometrium of cyclic rats

4

839/DEL/2015 26/03/2015

Novel herbal capsule (Pyodermacare-G) for treatment
of bacterial and mycotic dermatitis in canines

 

THRUST
AREAS OF RESEARCH

 

a.      
Pharmacological
and molecular characterization of ion channels and various receptors and
their
down-stream signaling pathways in buffalo myometrium;

b.     
Elucidation
of downstream signalling pathway(s) to membrane receptor(s) and ion
channels
involved in uterotonic and tocolytic effects of heavy-metals on buffalo
and rat
myometrium;

c.      
Biomonitoring
of metallic environmental pollutants in soil, water and plants and also
blood
of cows and buffaloes;

d.     
Effect of heavy metals and agrochemicals
on
biotransformation enzymes;

e.      
Ameliorative potential of medicinal
plants against
heavy metals and agrochemicals;

f.      
Pharmacokinetics
of antibacterial and non-steroidal anti-inflammatory drugs in ruminants;

g.      
Pharmacology
of Indigenous medicinal plants and development of herbal drug
formulations
against common ailments of milch and pet animals.

 

 

NEW
METHODS/TECHNIQUES
DEVELOPED

 

a.      
Rapid,
simple and consistent spectrofluorometric assay method for estimation of
ciprofloxacin
in blood;

b.     
Myometrial
preparations from buffalo
uterus for
demonstration of the effects of oxytocics, tocolytics, 
adrenoceptor and muscarinic receptor
agonsits, and demonstration of the effect of various potassium channel
openers,
blockers and different calcium regulatory/signalling pathways;

c.      
A simple extractionless reverse phase
HPLC method
for detection and quantification of cypermethrin in rat plasma

d.     
A simple extractionless reverse phase
HPLC method
for detection and quantification of flubendiamide in rat plasma

e.      
Novel non-medicated experimental model for pharmacodynamic
studies on rat
myometrium

 

NOTABLE
RESEARCH
HIGHLIGHTS

a.      
The transcript sequence of alpha subunit of
maxi potassium channels of 1097 bp length (NCBI EU816373) and ~100 kDA
protein
in non-pregnant buffalo myometrium.

b.     
The transcript sequence of histamine H1 receptors 311 bp length (NCBI KJ150294) and 57 kDA
protein in non-pregnant buffalo myometrium.

c.      
Molecular characterization of H2 histaminergic
receptors and TRP ion channels in buffalo myometrium.

d.     
Molecular characterization of alpha subunit of Na+-K+-ATPase (110 kDA) in
sheep pulmonary artery and modulation of alpha
subunit of Na+-K+-ATPase by fatty acids in
ovine
pulmonary and coronary arteries.

e.      
Vasodilator activity of eicosapentaenoic acid and
docosahexaenoic acid in 5-HT contracted ovine pulmonary and coronary
arteries.

f.      
The cellular coupling of beta2-adrenoceptors
with
potassium channels especially KATP and BKCa in
non-pregnant
buffalo myometrium.

g.      
Elucidation of extracellular and intracellular calcium
and downstream calcium signaling cascades in histamine, oxytocin and
prostaglandin-induced myometrial contraction in non-pregnant and
pregnant
buffaloes.

h.     
Elucidation of calcium-depending and calcium-mimicking
response of lead on
buffalo
myometrium

i.       
Bio-monitoring of heavy metals in
feed/fodder and soil
in Mathura district revealed presence of high levels of arsenic, cadmium
and
lead and health risk index showed that vegetation in the district does
not seem
to be safe for human and animal consumption.

j.       
Blood lead levels in cows and buffaloes in and around
Mathura were higher than the permissible limits

k.     
Augmentation of α1-adrenergic
response
in rats portal vein following
12 weeks
exposure
to lead
@ 2700 ppm
due to increased responsiveness of
receptors
along with augmented release of neurotransmitters from nerve endings.

l.       
Arsenic in combination with deltamethrin
potentiated
oxidative stress while in combination with chlorpyrifos, intensity was
lower
than their individual effects.

m.   
Arsenic and chlorpyrifos in combination
significantly decreased the activity of phase I and phase II
biotransformation
enzymes.

n.     
Ameliorative
potential of
Withania
somnifera
root extract (WRE) against lead-induced
augmentation of alpha1-adrenoceptors
possibly
by regulating catecholamines release from nerve endings
.

o.     
Withania
somnifera
roots extract and Moringa
oleifera
leaves extract showed promising ameliorative property
against
arsenic- and deltamethrin-induced toxicity.

p.     
Pharmacokinetic data of ofloxacin and
meloxicam in
yaks and cattle following different routes of drug administration and
computation of rational dosage regimens for these species of animals.

 


Faculty Members

Name

Designation Qualification Email Contact No. View
Dr.Satish
Kr.Garg
Professor & Head Ph D profsatish@gmail.com +919456054995
Dr.Rajesh
Mandil
Assistant Professor MVSc rajesh_mandil@rediffmail.com +919411295636
Dr.
Atul Prakash
Assistant Professor Ph D dratul2510@rediffmail.com +919997612314
Dr.
Rajkumar Singh Yadav
Assistant Professor MVSc rajan.vaday@gmail.com +918439490936
Dr.
Soumen Choudhury
Assistant Professor MVSc smnpharma@gmail.com +919411065114

 

LABORATORIES

 

  •    
    Molecular
    Pharmacology
    Laboratory
  •    
    Cardiovascular
    Pharmacology
    Laboratory
  •    
    Smooth
    Muscle
    Pharmacology Laboratory
  •    
    Ethnopharmacology
    Laboratory
  •    
    Microbial
    Assay
    Laboratory
  •    
    HPLC
    Laboratory
  •    
    GC-MS
    Laboratory
  •    
    Atomic
    Absorption
    Spectrophotometer Laboratory
  •    
    Neuropharmacology
    Laboratory
  •    
    Pharmacokinetic
    Laboratory
  •    
    Biochemical
    Pharmacology
    & Toxicology Laboratory
  •    
    Fluorescent
    Microscopy
    Laboratory

 

The
department is having well
equipped laboratories with major equipments/instruments like:

1.     
Data
acquisition system based physiograph

2.     
Millar
pressure transducer

3.     
Spectrofluorometer

4.     
Plethysmometer

5.     
Hot-cold
plate

6.     
Analgesiometer

7.     
Aggressometer

8.     
Pole
climbing apparatus

9.     
Rota-rod

10. 
Soxhlet apparatus

11. 
Rotary vacuum
evaporator

12. 
BOD incubator

13. 
Biosafety cabinet etc

14. 
Lyophilizer

15. 
Capsule and tablet
punching machine

16. 
Gas Chromatography-Mass
Spectrometry

17. 
Myograph

18. 
Fluoride Meter

19. 
Fluorescent Microscope

20. 
Nanodrop

21. 
Microdigestion system

Further, CIL with
sophisticated equipments like

1.     
High
Performance Liquid Chromatography

2.     
Atomic
Absorption Spectrometer

3.     
Spectrophotometer

4.     
Thermocycler

5.     
Gel
Documentation System

6.     
ELISA
reader

7.     
Ice
Flaking Machine


Coming soon ........

Achievements

RESEARCH
AWARDS

Ram Lal Agarwal National Award (2014) for outstanding research work on indigenous
medicinal plants (Prof Satish K.
Garg
and Dr Chandrabhan Bharshiv
)

 

Ram Lal Agarwal National Award (2011) for outstanding research work on indigenous
medicinal plants (Prof Satish K.
Garg,
Rajesh Mandil and Dr M. Jayanthi
)

 

Jawahar
Lal
Nehru Award of ICAR (2010)

for
best PhD Thesis entitled “Molecular characterization of Na+-K+-ATPase
isoforms
and their modulation by fatty lipids in ovine pulmonary and coronary
arteries”

 

Prof. Natarajan Young Scientist Award (2013)  to a MVSc student

 

Prof. V.V. Ranade Young Scientist Award (2008) to a PhD student

 

Best Poster Presentation Awards of ISVPT – 2001, 2002, 2009, 2012, 2014

DBT
and
CSIR, Govt. of India
 2009 Foreign Travel Grant for attending the 46th Congress of European Societies of Toxicology
held at
Dresden, Germany (
Dr Atul Prakash,
Assistant Professo
r)

 

BEST
TEACHER AWARDS

ICAR
Sponsored
Best Teacher Award of the University
(2012) to Prof. Satish Kumar Garg, Professor
and Head

 

Best
Teacher
Award of the University

(2004)
to Prof. Satish Kumar Garg, Professor and Head

 

STANDING
IN PROFESSIONAL SOCIETIES

Dr.
Satish
K. Garg, Professor & Head, President of Society of Toxicology (2014-2016).


Dr.
Satish
K. Garg, Professor & Head, Secretary
General
of Society of Toxicology (
1999-2001 and 2001- 2003).

Dr.
Satish
Kumar Garg, Professor and Head Secretary
General of Indian Society of Veterinary Pharmacology and
Toxicology (2003-2005,
2005-2007)

 

BOOKS
PUBLISHED/CHAPTERS
CONTRIBUTED

 ·                    
Veterinary
Toxicology (2000)
by Satish Kumar Garg. CBS
Publishers and
Distributors, New Delhi.

·                    
Chapter in the book entitled Selected Topics
on Camelids
by Satish Kumar
Garg.
(T.K. Gahlot,
Editor). First Edition. Camelid Publishers, Bikaner.

 ·         
    
Chapters in the book entitled “Text
Book
of Veterinary Pharmacology  &
Therapeutics”

(B.K Roy, editor). First Edition Kalyani Publishers, Ludhiana/New Delhi.

 ·                    
Satish
K. Garg
and
M. Sabir (2004). Nature of adrenoceptors in myometrium of ruminants – an
update. In: Reproductive Biology by R Mathur, Asha Mathur and Sangeeta
Shukla (eds). Deep and Deep Publications Pvt. Ltd., New Delhi.

 

 CONFERENCES/SEMINARS/TRAININGS/WORKSHOPS
ORGANIZED

 

S.
No.

Title

1

VIII
Annual Conference of Indian Society of Veterinary Pharmacology
and Toxicology (ISVPT) and National Symposia on Challenges,
scientific validation and IPR Protection of indigenous medicinal
plants based ITK and Emerging risks to wildlife due to drugs and
toxicants and ameliorative measures ” (November 6-8, 2008)

2

Pre-Conference
Workshop (Wet Lab) on “Canine echocardiography and telemetry”
accredited by AVMA for CE credits with
100% resource persons from USA (February 15-16, 2011).

3

IV International Conference of 
Laboratory Animals Scientists Association (LASA) on  “The Challenges ahead”
(February 17-18, 2011)

4

National
Seminar on “Livestock Policy for National Food and Nutritional
Security in the   of
WTO Regulations” and 11th Annual Convocation of
National Academy of Veterinary Sciences (India) (November 2-3,
2012)

5

33rd
Annual Conference of Society of Toxicology (India) and national
symposia on -----  (October
23-25, 2013).

6

Hands
on training on “Use of Data Acquisition System Based Physiograph
in Pharmacodynamic Studies” under ICAR Niche Area of excellence
Programme (March 9-13, 2015)